Dye degradation by fungi pdf

The retention times of the peaks of berberine 1, palmatine 2, alizarin 3, purpurin 4, indigotin 5, and indirubin. Jun, 2012 the uv spectrum confirms the decolourization. Phugare ss, kalyani dc, patil av, jadhav jp 2010 textile dye degradation by bacterial consortium and subsequent toxicological analysis of dye and dye metabolites using cytotoxicity, genotoxicity and oxidative stress studies. A fixed amount of each dye was used in the culture medium to screen out the potential of the fungus for dye degradation. The outcome of the study contributes in taking microbial. Discoloration and biodegradation of two dyes by whiterot. Nov 20, 2014 berberine, palmatine, alizarin, purpurin, indigotin, and indirubin which were the major coloring compounds of phellodendron bark, madder, and indigo plant were thermally degraded in 100c oven in liquid dye form and also in silk dyed with five of these pigments. Decolorization of dye containing wastewater by fungi. Hence, it was concluded that ganoderma lucidum could be utilized for the effective treatment of textile dye wastewater.

Studies and research in zoology, scott christian college autonomous, nagercoil 629 003, kanyakumari district, tamil nadu, india key words. In this study we intended to isolate and identify azo dye degrading and decolorizing bacterial strains as well as investigation its biodegradation mechanism. Real dye containing wastewater samples were added to fungi and its effectiveness in dye color removal evaluated. Hplc analysis of the intermediates and end products from dye degradation showed that the decolorized dyes by the in vitro systems could still have residual fragments such as aromatic rings which had high uv adsorption. Fungal degradation of the bioplastic phb poly3hydroxy. Degradation of azo dyes by trametes villosa laccase over. Effect of agitation on dye degradation the effect of agitation on dye degradation was determined by incubating msm broth containing fungi at various rpm revolution per minute like 20,40,60,80,100 for 24 hrs. Chacko, kalidass subramaniam international journal of environmental sciences volume 1 no. Dye removal from wastewater is a difficult task and expensive2. By using certain fungi, eco friendly degradation of azo dyes can be carried out to remove toxicity.

Complete degradation of dye into nonharmful state or form is essential3. Biodegradation of reactive red m5b dye using bacillus subtilis. Oct 17, 2014 phugare ss, kalyani dc, patil av, jadhav jp 2010 textile dye degradation by bacterial consortium and subsequent toxicological analysis of dye and dye metabolites using cytotoxicity, genotoxicity and oxidative stress studies. Textile effluent, azo dye, congo red, decolourization, bio degradation, hplc.

The most widely explored fungi with regard to dye degradation are the ligninolytic fungi bumpus,1995. This is not necessary if you use an aluminum, tin, or copper pot as the metal in the pot will take part in the dyeing reaction. Results and discussion broadspectrum of decolorization most of fungi screened for decolorization were valid for limited dyes. White rot fungi were capable of secreting active ligninolytic enzymes and decolourising the pulp and paper mill. Ligninolytic enzyme and dye degradation activity was tested against azo tex. The goal of fungal treatment is to decolorize and detoxify the dye contaminated effluents. Singh and others published degradation of anthroquinone dyes stimulated by fungi find, read and cite all the research you need on researchgate. From the results, it was found that the degradation of textile dye wastewater follows firstorder kinetics. Therefore, the purpose of this work was the study of azo dye degradation products in the presence of laccase. Microbial decolourization and degradation is an eco. Fungal degradation of the bioplastic phb poly3hydroxybutyric acid k. Recently, several other white rot fungi have been shown to.

The decolorization of the dye takes place either by adsorption on the microbial biomass or and enzymatic degradation. Biodegradation of textile dyes, bromophenol blue and congored. Decolourization and degradation of dyes in industrial. Decolorization study showed that the methyl orange dye was removed by more than 50% in 3 days, eriochrome balck t removed by more than 33% and hplc analysis determined several degradation products.

Biodegradation and decolourization of textile dye wastewater. Analysis of bacterial degradation of azo dye congo red using hplc d. Table 2 decolourization of dye containing waste water using fungi. White rot fungi, coriolus versicolor, termetomyces sp, pleurotus ostreatus, schizophyllum commune, decolourization of azo dyes.

An analysis of the data reveals that out of four solid media used three did not show the degradation of dye by the growing microorganisms. Biodegradation of dyes using consortium of bacterial. Recent studies on the use of fungi and enzymes for bioremediation of azo dyes are surveyed. The percent dye degradation by the test isolate at different ranges of.

Degradation dyes lignindegrading fungus phanerochaete. Decolorization of azo, anthraquinone, heterocyclic, triphenylmethane and polymeric dyes by white rot fungus phanerochaete chrysosporium have been reported11,12. Microbial degradation of textile effluents were critically analysed and discussed. The capability of whiterot fungi to removal synthetic dye is due to extracellular enzyme production such as laccases, lignin peroxidases lip and manganese peroxidases mnp atteke et al. Bioremediation of dyes by fungi isolated from contaminated. While fungal treatment of dye containing effluents is usually time consuming and difficult to control24, the potential of enzymes for this purpose has clearly been demonstrated. Several other dye decolorizing fungal species have been reported, which include aspergillus niveus 2 ve fusarium moniliforme 8. Analysis of thermal degradation of mixed dye solution. Decolourization and degradation of dyes in industrial effluents by native endophytic fungi dr. Fungi offers cheaper and efficient alternative for decolorization or degradation of recalcitrant textile dyes.

Aspergillus niger recorded maximum decolorization of the dye basic fuchsin 81. Because these fungi grow into spheres in liquid culture, they can be counted to provide an. Furthermore, the fungi degraded these dyes without accumulating any phenolic compounds. Sixtythree local whiterot fungi were isolated from soil and wood samples on potato dextrose agar pda. Table 1 biodegradation of textile dyes, bromophenol blue and congo red by a. Isolation and screening of dye degrading microorganisms from. Degradation of azo dyes by trametes villosa laccase over long. The outcome of the study contributes in taking microbial dye remediation from laboratory to field conditions. These fungi have selected in present study because a. Bioremediation takes place by anaerobic andor aerobic process.

Biodegradation of textile dyes, bromophenol blue and. Screening of fungi for decolorization of dye wastewater ipcbee. Fungi dye decolourization % incubation period days references. The degradation and decolourization mechanism, but with white rot mineralization of dyes is successful by certain fungi both adsorption and degradation can.

The objective of this study was to assess the congo red biodegradation and detoxification by aspergillus niger. Decolorization of triphenylmethane dyes by six whiterot fungi. Substantial quantities of these dyes have been deposited in the environment, particularly in streams and rivers. Trametes villosa laccase was used for direct azo dye degradation, and the reaction products that accumulated after 72 h of incubation were analyzed. Various models were tried to study the kinetics of textile dye degradation. Thermal degradation of natural dyes and their analysis using. Microbial degradation and decolourization of dyes in semi. Aug 08, 2017 decolourization and degradation of dyes in industrial effluents by native endophytic fungi dr. Congo red decolorization and detoxification by aspergillus. In all cases, bacterial degradation is initiated bythe reduction ofthe azo corresponding author. Bacterial degradation of textile dyes springerlink. Fungal decolourization of direct azo dyes and biodegradation of textile dye effluentdecolourization degradation direct azo dyes textile dye effluent.

The inhibition of fungal growth in dyecontaining medium was also observed during dye degradation table 1. Azo dyes are used in a wide variety of products and can be found in the effluent of most sewage treatment facilities. Fungi dye decolourization % incubation period days references phanerochaetechrysosporium methyl violet, congo red, acid orange, acid red 114, vat magenta, methylene blue and acid green 8898 310 29 trametes coriolus versicolour reactive blue 4 50 mgl 98 16 30. Oct 09, 2014 results of the dye biodegradation by soil fungi in this study using spectrophotometric analysis were even comparable with the percent dye decolorization exhibited by the whiterot fungus trametes versicolor and pleurotus ostreatus yao et al. Decolorization of reactive dyes by trametes villosa ccb176 and pycnoporus sanguineus ufmgcb03 on malt extract agar containing 0. Hplcdadms analysis was initially conducted on the untreated dye solution to find the retention time of the chromatogram peak, uvvisible absorption spectrum. All the media used differs in the capacity to support the growth of.

Thermal degradation of natural dyes and their analysis. Huss1 phb poly3hydroxybutyric acid is a thermoplastic polyester synthesized by ralstonia eutropha and other bacteria as a form of intracellular carbon and energy storage and accumulated as inclusions in the cytoplasm of these. They were each mixed with 5 days old mycelia in roughly 2. Liquid chromatographymass spectrometry lcms analysis showed the formation of phenolic compounds during the dye oxidation process as well as a large amount of polymerized products that retain azo group integrity. Introduction the two major sources of dye release into the environment are the textile and dyestuff manufacturing industries1. Among the advanced oxidation processes, photocatalysis by semiconducting materials has become a leading technology in the field of environmental cleaning. This is not necessary if you use an aluminum, tin, or copper pot as. Screening of soilderived fungi for dye decolorization activities. Different methods employed in dye removal include membrane filtration, coagulation, flocculation, precipitation, floatation, adsorption, ionexchange. The most potent isolate was identified as pseudomonas spp via partial sequencing of 16s. However, lasparagine was used as the original nitrogen source and it was known that amino acid could also be the source of carbon for fungi.

Synthetic dye degradation by whiterot fungi and fungal. However, contrary to the performance shown in aseptic batch tests, the application of white rot fungi in continuous bioreactors for dye wastewater treatment has been so far impeded by problems such as excessive growth of fungi causing reactorclogging zhang et al. Decolourization of dye wastewater by microbial methods a. Direct azo dye laccase degradation and aminophenol polymerization were performed for several days. The extent of degradation for these dyes by fungi can be evaluated with the help of uvvisible spectrophotometer and high performance liquid chromatography. Biodegradation of dyes by basidiomycetes fungi using hplc and. All the media used differs in the capacity to support the growth of dye degrading microorganisms. Results of the dye biodegradation by soil fungi in this study using spectrophotometric analysis were even comparable with the percent dye decolorization exhibited by the whiterot fungus trametes versicolor and pleurotus ostreatus yao et al. Pdf bioremediation of textile dye using white rot fungi. The mechanism of fungal decolouration occurs from adsorption, enzymatic degradation or a combination of both. By using certain fungi, eco friendly degradation of azo dyes can be carried out to remove toxicity and thus can help in keeping the environment free from pollution.

The presence of living fungal cells, however, showed a better extent of dye degradation than that of in vitro systems. Biomass degradation refers to the utilization of microorganisms such as bacteria and fungi to produce enzymes that can interact with molecules of dyes. Analysis of bacterial degradation of azo dye congo red. Congo red is one of the best known and used azo dyes which has two azo bonds nn chromophore in its molecular structure. A degradation pathway has been proposed by champagne and ramsay 2005 in which the azo bond may be cleaved first martins et al. Its structural stability makes it highly toxic and resistant to biodegradation.

The result of the dye degradation by the isolate is shown in figure 1af. Several medium factors can influence the ability of fungi in biodegradation, such as temperature, concentration of dye, ph and the chemical structure of dye. Bioremediation of dyes by fungi isolated from contaminated dye. Degradation of drimaren brilliant blue the content of the flasks was removed at determined time intervals and filtered 0. Biodegradation of dyes using consortium of bacterial strains. Biodegradation of dyes by basidiomycetes fungi using hplc. The ability to decolorize three triphenyl methane dyes crystal violet, bromophenol blue and. Data is presented showing how various factors affect enzymatic azo dye degradation. Pdf growth, dye degradation and ligninolytic activity. Pdf 38 biodegradation of azo dyes by fungi researchgate. Among these basdiomycetes are the best producers of laccase. Microbial degradation of dyes by the fungus trichoderma harzianum, along with the effects of these dyes on this fungus during their biodegradation, was investigated using semisolid medium pda, himedia. Degradation of azo dyes by environmental microorganisms and.

Discoloration and biodegradation of two dyes by whiterot fungi. With the same interest, present study focuses on exploration of dye degradation efficiency of three different pleurotus sp. Pdf biodegradation of textile dyes, bromophenol blue and. Existing physicalchemical technologies for colour removal are very expensive. Natural dyeing normally requires a color fixative such as alum. The lignindegrading white rot fungus phanerochaete. Decolorization pathways of anthraquinone dye disperse blue. Characterization of an azodyedegrading white rot fungus. The inhibition of fungal growth in dye containing medium was also observed during dye degradation table 1. Pdf degradation of anthroquinone dyes stimulated by fungi. Several microorganisms, such as fungi, bacteria and yeasts, have ability to discolour and even completely transform many dyes under optimum conditions. Pdf on dec 17, 2003, john a bumpus and others published 38 biodegradation of azo dyes by fungi find, read and cite all the research you need on researchgate. Microbial degradation of azo dye by pseudomonas spp 24. Phenol kills the fungi, and the lactic acid increases preservation chino cotton blue is a stain for chitin and cellulose.

Various approaches using fungalenzymatic degradation are summarized. Growth, dye degradation and ligninolytic activity studies on zimbabwean white rot fungi. White rot fungi have emerged as promising organism for treating color from wastewater9,10. Pdf biodegradation and detoxification of dyes, malachite green, nigrosin and basic fuchsin have been carried out using two fungal isolates. Different bacterial isolates were isolated from activated sludge of common effluent treatment plant and tested against reactive violet 5 rv5 dye. The isolated bacteria were capable of decolorizing 0. Joel gnanadoss et al bioremediation of textile dye using white rot fungi.

Isolation and screening of dye degrading microorganisms. The initial recognition of white rot fungi competency in decolorization lays the foundation for its application in dye degradation. Role of ligninolytic enzymes of white rot fungi pleurotus. Learners discover that natural chemicals in fungi can dye wool different colors. According to our uv spectroscopic methods, biodegradation of dyes occur by the fungi used in the kirks medium. Screening of fungi for decolorization of dye wastewater. The present study deals with the degradation of methyl red mr and carbol fuchsin cf by bacteria and its consortium isolated from textile effluents. Sixty one dye effluent soil fungal strains were screened for their ability to degrade.

The fungi were also grown in culture medium without the synthetic effluent. This feature is probably due to the ability of white rot fungi. Degradation of azo dyes by environmental microorganisms. The effects of ph, initial dye concentration, temperature, and shaking. Pdf bioremediation of dyes by fungi isolated from contaminated.